Showing posts with label animals and plants. Show all posts
Showing posts with label animals and plants. Show all posts

Soil Microorganisms - The World You Can't See

Building high populations of beneficial microorganisms in the soil will dramatically increase growing success, soil and plant health, and help to increase satisfaction as a grower. Microorganisms are one major piece of the gardening puzzle. Microbes convert nutrients into (bio-available) forms that plants can use, including fixing nitrogen. A high percentage of nutrients applied to soils go unused, wasting valuable nutrients leading to groundwater contamination in areas with high water tables and wasting money. Microbes also produce enzymes and hormones that are needed by plants to grow. Additionally, beneficial microbes are able to attack pathogenic microbes and breakdown many of the toxic substances they produce. Microbes are great to apply directly to the soil and on the plant as part of a foliar feeding program. However, they are very misunderstood. This is very likely due to the fact that most people think of microbes or bacteria as bad because they associate them with sickness. However, without microbes we would not be able to live healthy lives.

Microbes include bacteria, viruses, fungi, algae, yeast, amoebas, protozoa, nematodes, arthropods, insects, and earthworms. There are aerobic and anaerobic kinds. This means some require lots of air (aerobic) and other do not (anaerobic). In general people tend to think of anaerobic bacteria as pathogens and aerobic bacteria as beneficial, or non-pathogenic. However, there are pathogenic aerobic microbes and not all anaerobic microbes are pathogenic. Pathogens are called such because they produce various substances that can be toxic to humans, animals, and plants. Pathogens are generally associated with a putrefactive (rotting) pathway. The by-products of putrefaction are odorous and very often toxic to plants, humans and animals.

Microbes can be introduced in a variety of ways. These include making compost teas, buying inoculants such as such as EM•1 Microbial Inoculant, that contain a diverse species from several genera, and beneficial fungi such as mycchorizae. There is a trick when adding microbes though. There is a lot of mass and billions of microbes in the soil. It takes regular applications with very high populations of microbes and good land management to make changes. When the numbers of microbes from a inoculant are significant and certain methods are followed, they are able to suppress the growth of pathogens including the above-mentioned bacteria and microbes such as E. coli, Salmonella, Botulinum, etc. This process is also known as competitive exclusion and is very commonly practiced in food manufacturing.

Carbohydrates are food for the microbes and are converted to various beneficial compounds by lactic acid bacteria and yeasts such as trace minerals, vitamins, amino acids, and enzymes, which are utilized by other microbes and macrobes (worms and other insects). A typical conversion of sugar under the lactic acid bacteria type of fermentation will follow the following formulas:

C6H12O6 → CH3CHOHCOOH + C2H5OH + CO2

C6H12O6 → 2 CH3CHOHCOOH

Lactic acid bacteria and yeast both produce small amounts of carbon dioxide (CO2), Oxygen (O2), and hydrogen (H2). However, during anaerobic phases, the Lactic acid bacteria will not produce gas and the yeast will produce carbonic acid instead of alcohol. Several species of photosynthetic microbes grow and increase their biomass by absorbing carbon dioxide, but can also increase biomass by degrading organic compounds including such toxic compounds as 3chlorobenzoate to cellular building blocks. When oxygen is present, R. palustris generates energy by degrading a variety of carbon-containing compounds (including sugars, lignin monomers, and methanol) and by carrying out respiration. ( http://genome.jgi-psf.org/rhopa/rhopa.home.html )

Anaerobic/pathogens produce methane, butyric acid, Hydrogen Sulfide, Ammonia, and promote the growth of pathogenic species. Aerobic/beneficials produce or release oxygen, hydrogen, carbon dioxide, enzymes, amino acids, trace minerals, bio-available vitamins, ammonium, and promote the growth of other beneficial microorganisms.

As you can see, there is a lot going on with microbes. And, this is only a small look into the world of microbes. With the billions of microbes that are in soil, we want to make sure the soil maintains high levels of beneficial microbes to promote healthy soils and plants. This is done through inoculating the soil with various amendments and good management of the soil. It can also be helped along with adding in various inoculants containing high populations of beneficial microorganisms and a diversity of species. From this we can also see the importance of preventing compacted (anaerobic) soils as most pathogens are anaerobic.

As one becomes a biological gardener/farmer, they tend to use their senses more and observations are used to address issues. Watch for compaction. Look at the surface after watering or heavy rains. See which areas drain quickly. Watch plants to see areas that tend to get attacked by pests and/or disease. Look for worms in the soil. Looks for pests and predators. As you explore this new world, think about what you cannot see and think about the billions of microbes in the soil that hard at work to make the nutrients you add available for you plants and how they affect the soil structure. Give them a little help by regularly sending in some reinforcements. Over time you will notice changes in the soil, drainage, plant health, pest infestation, and find that fruits and vegetables get sweeter and sweeter year after year.

Eric Lancaster is Executive Vice President of TeraGanix, Inc., the exclusive North America distributor of Effective Microorganisms® and EM® Bokashi products. He has been using the Effective Microorganisms® at home and commercially for 14 years. Please visit http://www.TeraGanix.com for more information.

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How Modern Technology Has Transformed Microscopy

The simplest form of magnification is ironically a drop of water. It makes a perfect lens. Have you ever noticed the type of a magazine or newspaper while a drop of water rests on it? The type face becomes bolder, or magnified, due to the refraction of light coming back through the clear drop of water.

Refraction is the key to how lenses work. A lens made of crystal or glass can be concave or convex. The concave lens tends to refract light so that an image is smaller when viewed through it. A convex lens will make an object larger. The culmination of lens size and conglomeration of lens type can make a telescope or microscope. In this article we will look at the advances in lens design and application that has sent microscopy into the future.

The first lenses were accidental. Orbs of glass were made for ornamental reasons, but when placed over objects, the observer noticed them becoming larger. Also, the sun could be focused to a point that would ignite parchment or wood. This focusing of light was easily observed, thus sparking the interest of the thinking minds of that age to develop further.

Galileo is known as the Father of the Telescope, but he tinkered with lenses for microscopy as well. His lenses were hand ground to his specifications, yet his specifications were trial and error. The advent of his telescope revealed the planets and even the moons of Jupiter, and this became his passion, not microscopy. Yet the process of grinding and polishing lenses was handed down to the next generation of microscope builder.

Anton van Leeuwenhoek developed his own type of lens, which was spherical. His microscopes enabled him to view extremely tiny living creatures, called protozoa (single-celled animals and plants), yet he named "animalcules." He could see blood cells and yeasts as well, making him the Father of Microscopy.

The grinding and shaping of lenses was perfected by the American Charles A. Spencer, his design being used by physicians and the new field of microbiology. This new design had convex and concave lenses used in unison to bring more brightness and eye relief to the microscope. Magnification now became the priority of microscope developers.

Coated lenses were the next step. The special coatings enabled light to carry along the natural colors of the subject without any apparent aberrations, thus making a view that was bright and clear. Focusing controls were streamlined for better precision.

The first light microscope with separate low - to - high power objectives was the obvious next step, enabling a specimen to be view in a more panoramic low power, to an intense high power mode. Details never before realized were now in the possession of modern day researchers in both the medical and biological fields. Metallurgical microscopy was now easier, and geology had a new frontier.

The latest in technology is the invention of the Electron Microscope. Instead of lenses made of glass or crystal, this powerful instrument uses magnets to focus a beam of electron bouncing off of the specimen. This enables research scientists to see objects nearly at the molecular level, so that even DNA cannot hide from our eyes.

Technology is ever-advancing; the next microscope may be one that actually sees individual protons and electrons in and atom. This would be a marvel of science, leading to the fabrication of new and fascinating materials.

Take a look at other relevant information from Andrew Long about microscopy related products including confocal microscope and laboratory microscopes [http://www.laboratorymicroscopes.org]

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